LOCUS       40924_10686        7564 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCHR8 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Miyazaki C, Saito A.
  TITLE     Construction of a rice bacterial artificial chromosome library using
            a new vector suitable for map-based cloning and identification of 
            clones linked to lethal factor locus in rice chromosome 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Miyazaki C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-1998) Contact:Chikara Miyazaki Kyushu National 
            Agricultural Experiment Station, Department of Crop Breeding; 2421 
            Suya, Nishigoshi, Kumamoto 861-1192, Japan
REFERENCE   3  (bases 1 to 7564)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7564)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JUN-1998) Contact:Chikara Miyazaki Kyushu National Agricultural 
            Experiment Station, Department of Crop Breeding; 2421 Suya, 
            Nishigoshi, Kumamoto 861-1192, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     289..305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        312..330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    333..440
                     /label=multicloning site from EcoRI to MluI
                     /note="multicloning site from EcoRI to MluI"
     promoter        complement(453..471)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(489..505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(513..529)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(537..567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(582..603)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(826..1482)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1483..1585)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2512..2731
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2822..3574
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3580..3830
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             4156..5328
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             5331..6299
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    6375..6848
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(7108..7506)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(7524..7557)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
      181 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      241 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      301 ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cgggggcgcg
      361 ccggatcctt aattaagtct agagtcgatt taaatgactg tttaaacctg caggcatgca
      421 agcttgggct agctacgcgt gagcttgagt attctatagt gtcacctaaa tagcttggcg
      481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      661 taatgaatcg gccaacgcga accccttgcg gccgcccggg ccgtcgacca attctcatgt
      721 ttgacagctt atcatcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta
      781 gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
      841 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
      901 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
      961 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1021 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     1081 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     1141 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     1201 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     1261 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1321 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     1381 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     1441 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     1501 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1561 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt
     1621 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     1681 tatttattcg cgataagctc atggagcggc gtaaccgtcg cacaggaagg acagagaaag
     1741 cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gattggggag gcggttgccg
     1801 ccgctgctgc tgacggtgtg acgttctctg ttccggtcac accacatacg ttccgccatt
     1861 cctatgcgat gcacatgctg tatgccggta taccgctgaa agttctgcaa agcctgatgg
     1921 gacataagtc catcagttca acggaagtct acacgaaggt ttttgcgctg gatgtggctg
     1981 cccggcaccg ggtgcagttt gcgatgccgg agtctgatgc ggttgcgatg ctgaaacaat
     2041 tatcctgaga ataaatgcct tggcctttat atggaaatgt ggaactgagt ggatatgctg
     2101 tttttgtctg ttaaacagag aagctggctg ttatccactg agaagcgaac gaaacagtcg
     2161 ggaaaatctc ccattatcgt agagatccgc attattaatc tcaggagcct gtgtagcgtt
     2221 tataggaagt agtgttctgt catgatgcct gcaagcggta acgaaaacga tttgaatatg
     2281 ccttcaggaa caatagaaat cttcgtgcgg tgttacgttg aagtggagcg gattatgtca
     2341 gcaatggaca gaacaaccta atgaacacag aaccatgatg tggtctgtcc ttttacagcc
     2401 agtagtgctc gccgcagtcg agcgacaggg cgaagccctc gagtgagcga ggaagcacca
     2461 gggaacagca cttatatatt ctgcttacac acgatgcctg aaaaaacttc ccttggggtt
     2521 atccacttat ccacggggat atttttataa ttattttttt tatagttttt agatcttctt
     2581 ttttagagcg ccttgtaggc ctttatccat gctggttcta gagaaggtgt tgtgacaaat
     2641 tgccctttca gtgtgacaaa tcaccctcaa atgacagtcc tgtctgtgac aaattgccct
     2701 taaccctgtg acaaattgcc ctcagaagaa gctgtttttt cacaaagtta tccctgctta
     2761 ttgactcttt tttatttagt gtgacaatct aaaaacttgt cacacttcac atggatctgt
     2821 catggcggaa acagcggtta tcaatcacaa gaaacgtaaa aatagcccgc gaatcgtcca
     2881 gtcaaacgac ctcactgagg cggcatatag tctctcccgg gatcaaaaac gtatgctgta
     2941 tctgttcgtt gaccagatca gaaaatctga tggcacccta caggaacatg acggtatctg
     3001 cgagatccat gttgctaaat atgctgaaat attcggattg acctctgcgg aagccagtaa
     3061 ggatatacgg caggcattga agagtttcgc ggggaaggaa gtggtttttt atcgccctga
     3121 agaggatgcc ggcgatgaaa aaggctatga atcttttcct tggtttatca aacgtgcgca
     3181 cagtccatcc agagggcttt acagtgtaca tatcaaccca tatctcattc ccttctttat
     3241 cgggttacag aaccggttta cgcagtttcg gcttagtgaa acaaaagaaa tcaccaatcc
     3301 gtatgccatg cgtttatacg aatccctgtg tcagtatcgt aagccggatg gctcaggcat
     3361 cgtctctctg aaaatcgact ggatcataga gcgttaccag ctgcctcaaa gttaccagcg
     3421 tatgcctgac ttccgccgcc gcttcctgca ggtctgtgtt aatgagatca acagcagaac
     3481 tccaatgcgc ctctcataca ttgagaaaaa gaaaggccgc cagacgactc atatcgtatt
     3541 ttccttccgc gatatcactt ccatgacgac aggatagtct gagggttatc tgtcacagat
     3601 ttgagggtgg ttcgtcacat ttgttctgac ctactgaggg taatttgtca cagttttgct
     3661 gtttccttca gcctgcatgg attttctcat actttttgaa ctgtaatttt taaggaagcc
     3721 aaatttgagg gcagtttgtc acagttgatt tccttctctt tcccttcgtc atgtgacctg
     3781 atatcggggg ttagttcgtc atcattgatg agggttgatt atcacagttt attactctga
     3841 attggctatc cgcgtgtgta cctctacctg gagtttttcc cacggtggat atttcttctt
     3901 gcgctgagcg taagagctat ctgacagaac agttcttctt tgcttcctcg ccagttcgct
     3961 cgctatgctc ggttacacgg ctgcggcgag cgctagtgat aataagtgac tgaggtatgt
     4021 gctcttctta tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga
     4081 tgactttgcg attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa
     4141 agcaatgatt aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac
     4201 gctggtcatg aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa
     4261 gcgaggaaaa taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct
     4321 tctcaggcta tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt
     4381 cgaggacggg ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat
     4441 gtgtttggta cgcgattgcg acgtgctgaa gacgtatttc caccggtgat cggggttgct
     4501 gcccataaag gtggcgttta caaaacctca gtttctgttc atcttgctca ggatctggct
     4561 ctgaaggggc tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agcctcaatg
     4621 tatcacggat gggtaccaga tcttcatatt catgcagaag acactctcct gcctttctat
     4681 cttggggaaa aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac
     4741 attattcctt cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat
     4801 gaaggtaaac tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct
     4861 catgactatg atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat
     4921 gtcgtatgtg ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc
     4981 tccgcactgc agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg
     5041 ttcgagcctg atgtacgtat tttgcttacc aaatacagca atagtaatgg ctctcagtcc
     5101 ccgtggatgg aggagcaaat tcgggatgcc tggggaagca tggttctaaa aaatgttgta
     5161 cgtgaaacgg atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc
     5221 attgatcaac gctcttcaac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc
     5281 tgcaatgaaa ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg
     5341 cgcctgttat tccaaaacat acgctcaata ctcaaccggt tgaagatact tcgttatcga
     5401 caccagctgc cccgatggtg gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta
     5461 atgccattac tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg
     5521 gtgatagtgt tgagaagacc tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc
     5581 ttactgagga cgcactggat gatctcatcc cttcttttct actgactggt caacagacac
     5641 cggcgttcgg tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta
     5701 aagctgctgc acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc
     5761 agatggctgc attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg
     5821 gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg
     5881 atgcggaaaa tatttcacgt aagattatta cccgctgtat caacaccgcc aaattgccta
     5941 aatcagttgt tgctcttttt tctcaccccg gtgaactatc tgcccggtca ggtgatgcac
     6001 ttcaaaaagc ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg
     6061 agcagaaaaa agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg
     6121 tgcttaaaac gtcatctgca tcaagaacta gtttaagctc acgacatcag tttgctcctg
     6181 gagcgacagt attgtataag ggcgataaaa tggtgcttaa cctggacagg tctcgtgttc
     6241 caactgagtg tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct
     6301 gatgcgacca cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc
     6361 cagaaagcat aactggcctg aatattctct ctgggcccac tgttccactt gtatcgtcgg
     6421 tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     6481 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     6541 tatcgtcggt ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtctgatta
     6601 ttagtctggg accatggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg
     6661 tcccactcgt atcgtcggtc tgattattag tctggaacca cggtcccact cgtatcgtcg
     6721 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg
     6781 ggaccacgat cccactcgtg ttgtcggtct gattatcggt ctgggaccac ggtcccactt
     6841 gtattgtcga tcagactatc agcgtgagac tacgattcca tcaatgcctg tcaagggcaa
     6901 gtattgacat gtcgtcgtaa cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct
     6961 gctgtggatt gctgctgtgt cctgcttatc cacaacattt tgcgcacggt tatgtggaca
     7021 aaatacctgg ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt
     7081 gtcgctgtcg acgagctcgc gagctcggac atgaggttgc cccgtattca gtgtcgctga
     7141 tttgtattgt ctgaagttgt ttttacgtta agttgatgca gatcaattaa tacgatacct
     7201 gcgtcataat tgattatttg acgtggtttg atggcctcca cgcacgttgt gatatgtaga
     7261 tgataatcat tatcacttta cgggtccttt ccggtgatcc gacaggttac ggggcggcga
     7321 cctcgcgggt tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc
     7381 gtcataactt aatgttttta tttaaaatac cctctgaaaa gaaaggaaac gacaggtgct
     7441 gaaagcgagc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca
     7501 atggaagtcc gagctcatcg ctaataactt cgtatagcat acattatacg aagttatatt
     7561 cgat
//
